Limna - Lausanne Integrative Metabolism Nutrition Alliance

PAST EVENT: SEMINAR

Friday 26th June 2015
12h15 - Department of Physiology, Bugnon 7, 1005 Lausanne Seminar room, 6th floor

HIGHLY EFFICIENT ESTABLISHMENT OF CONDITIONAL KNOCKOUT CELL LINES BY CRISPR-CAS9-MEDIATED GENOME ENGINEERING


SEMINAR

Dr. PATRICIA RENCK NUNES

Swiss Institute for Experimental Cancer Research, School of Life Sciences, EPFL, Lausanne

The CRISPR-­Cas9 system is a bacterial adaptive immune system recently adjusted to serve as a powerful tool for genome-­editing. Applying simultaneously the Cas9 endonuclease, a CRISPR-derived guiding RNA and a DNA repair template, it is possible to target and modify any genomic  sequence  by  homologous recombination. On top of that, Cas9 fusion proteins can be used to  activate  or  repress  the  expression  of different genes of the human genome. Our laboratory currently applies CRISPR targeting and large-­scale proteomics to identify genes playing a role in aging, cancer and telomere biology.

Host: Prof. Lluis Fajas

Mali et al. Science. 2013. Ran et al. Nature Protocols. 2013. Gilbert et al. Cell. 2013